Creative Diagnostics Launches Assay Service to Reduce Virus Yield

Creative Diagnostics Launches Assay Service to Reduce Virus Yield

Facebook
Twitter
LinkedIn

As an expert in virology and microbiology, Creative Diagnostics recently presented the Assay service to reduce virus yield Helping researchers evaluate the antiviral activity of compounds with the goal of simplifying the testing process and significantly reducing the workload for customers.

Various techniques are currently used to assess the antiviral activity of compounds. Some popular methods include plaque reduction, dye uptake, nucleic acid hybridization, and enzyme-linked immunosorbent assays. Less common is the Virus Yield Reduction Assay, which incubates a virus-infected culture with antiviral compounds for a period of time, allows the virus to replicate, and then titrates new native viruses with separate monolayer cultures.

Although the Virus Yield Reduction Assay allows for a quantitative measurement of the production of infectious virus particles in drug-treated cultures, the labor-intensive nature of this assay has so far prevented its widespread use as a routine technique. Creative Diagnostics now offers full Virus Yield Reduction Assay services, utilizing microtitration technology to streamline the testing process and significantly reduce the required operations.

Benefits of the Yield Reduction Assay:

  • The multiplicity of infection (MOI) of the virus is high enough to infect about 100% of the cells.
  • The effect of attenuating antiviral activity during intracellular replication of the virus is minimized.
  • High accuracy by titrating progeny viruses in cell types that allow viral plaques.

characteristics

  • Enables high throughput processes.
  • Assess virus replication inhibition more reliably.
  • Allows more profound drug effects to be measured by using a higher MOI and quantifying primary virus, thereby determining a wider range of antiviral activity (1-106 PFU/mL).

These assays are performed in three main steps, ie infection of cells in the presence of various concentrations of the test substance; Collection of cells or…

.



Source story

More to explorer